Numaferm TEV protease, 6x His-Tag
Numaferm’s TEV protease is an improved variant of the Tobacco Etch Virus (TEV) wildtype enzyme. It harbors various beneficial mutations i.e. at T17, N68 and I77 for increased solubility and stability, and at S219 for auto-proteolysis protection compared to the wildtype enzyme. The recognition site of the Numaferm TEV protease is ENLYFQ/S (or G) and is inserted between your target protein (being located at the C-terminus) and the overhang to be removed (being located at the N-terminus). Order
Cleavage occurs between Q and S (or G). If your target carries an N-terminal S (or G), just insert ENLYFQ without S (or G) and your target is released native with the correct amino acid at the N-terminus. If your target carries an alternative amino acid, insert ENLYFQS (or G) into your construct. After the cleavage reaction, your target carries an additional S (or G) at its N-terminus.