Numaswitch™

Switch on the potential of inclusion bodies
Make use of Numaswitch for efficient inclusion body-based production processes for the first time ever. Nowadays, inclusion bodies (aggregated proteins formed during expressions of target peptides and proteins) are often regarded as waste during recombinant production, despite being expressed in high-titers. This is due to challenges associated with the refolding step (<25% efficiencies).
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The Switchtag is a nature-evolved sensor reacting towards Ca2+ ions

Switch on the potential of inclusion bodies
In the absence of Ca2+ ions the Switchtag has an unstructured conformation that forces inclusion body formation (aggregates.) In presence of Ca2+ ions the Switchtag folds into a stable, water-soluble functional protein.

Aggregation

The OFF-state

During expression inside E. coli cells (Ca2+ (arrow downwards)) no Ca2+ ions bind to the Switchtag. This triggers the formation of inclusion bodies.

This is important to protect targets from proteolysis and degradation. It also protects E. coli cells from being harmed by targets.

Refolding

The ON-STATE

The inclusion bodies are extracted, solubilized in a denatured form and incubated with a dedicated refolding buffer containing high concentrations of Ca2+ ions. This induces the transformation of the aggregates (up to 100%) into highly stable, water-soluble and functional proteins. Now your target is released traceless by a protease.

Numaswitch enables up to 100%
refolding efficiences of inclusion bodies

Make use of Numaswitch for efficient inclusion
body-based production processes for the first time ever.
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