Numaswitch™

Switch on the potential of inclusion bodies

Numaswitch is changing the game, making inclusion bodies accessible. Often regarded as waste during recombinant production, inclusion bodies are expressed in high-titers making them revolutionary to the production of peptides and proteins at scale.

The Switchtag is a nature-evolved sensor reacting towards Ca²⁺ ions

Access the full power of recombinant processes

In the absence of Ca²⁺ ions the Switchtag has an unstructured conformation that forces inclusion body formation. In presence of Ca²⁺ ions the Switchtag folds into a stable, water-soluble functional protein.

Aggregation

The OFF-state

During expression inside E. coli cells (Ca2+ (arrow downwards)) no Ca2+ ions bind to the Switchtag. This triggers the formation of inclusion bodies.

This is important to protect targets from proteolysis and degradation. It also protects E. coli cells from being harmed by targets.

Refolding

The ON-state

The inclusion bodies are extracted, solubilized in a denatured form and incubated with a dedicated refolding buffer containing high concentrations of Ca²⁺ ions. This induces the transformation of the aggregates (up to 100%) into highly stable, water-soluble and functional proteins. Now your target is released traceless by a protease.

Numaswitch enables up to 100%
refolding efficiences of inclusion bodies

Make use of Numaswitch for efficient inclusion
body-based production processes for the first time ever.

Download our peer-reviewed article

An efficient high-titer expression platform to produce peptides and small proteins

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