Numacut TEV Protease,
6x His-Tag
The Numacut TEV protease is an improved variant of the Tobacco Etch Virus (TEV) protease with widened substrate tolerance. In comparison to the wild type enzyme, Numacut harbours various advantageous mutations. Primarily, instead of accepting the recognition sequence ENLYFQS (or G) and cleaving between Q and S/G (called P1' position), Numacut can be applied for all amino acids at P1', expect proline (P). In addition, further beneficial mutations increase the specific activity, solubility and stability outperforming the wildtype enzyme significantly. The N-terminal 6x His-Tag paves the way for easy and efficient removal of the Numacut from cleavage reactions by immobilized metal-ion affinity chromatography (IMAC). If you desire your candidate to be sequence-identical without traces, choose Numacut.
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